ZEISS
Center Screen Area / Image Containers - Display and Image Analysis
ELYRA 7
354
000000-2262-999
03/2019 V_02
6.17
FRET View
In the
FRET
View
type (Fig. 568), FRET data sets can be analyzed:
−
Data gained performing Acceptor Photobleaching
−
Data gained performing Sensitized Emission
The data sets are acquired setting up the imaging procedure manually.
To access the
FRET
View, load or acquire either a multichannel data set (minimum 3 channels) or a time
series with bleach event and click on the
FRET
tab.
Depending on the type of data the Image Display in the
FRET
View shows 3 panels after initial analysis:
the FRET image, the original data and the table with the analyzed data according to the method chosen.
Tables can always be saved or copied
to the clipboard by right-mouse clicking on the table
display!
Fig. 568
FRET Display of Image after Sensitized Emission analysis
After a Sensitized Emission analysis the table shows the following parameters (Fig. 568):
Region
: The identification numbers of regions assigned for analysis. Region zero refers to the whole
image.
Time
: When a time series is analyzed the time point is shown here.
D avg.
: Average intensity of the region in the donor channel. These values are influenced by the chosen
settings in the Settings Tab. They may therefore vary from values of the same regions in the Histo tab.
A avg
.: Average intensity of the region in the acceptor channel. These values are influenced by the
chosen settings in the Settings Tab. They may therefore vary from values of the same regions in the Histo
tab.
F avg.
: Average intensity of the region in the FRET channel. These values are influenced by the chosen
settings in the Settings Tab. They may therefore vary from values of the same regions in the Histo tab.