ZEISS
Center Screen Area / Image Containers - Display and Image Analysis
ELYRA 7
378
000000-2262-999
03/2019 V_02
6.18.4.2
Model-based Drift Correction
When
Apply
is pressed, the software identifies on behalf of a model linear drift on sample structures and
uses them to automatically correct for this drift. The correction vector calculated from the average of all
structures will then be applied to the SMLM image (
HR
channels).
Once
Apply
is pressed, the
Remove
button becomes available, when a
Model-Based
drift correction
has been performed (Fig. 623). Hence an inactive
Remove
button is indicative of a SMLM image without
Model-Based drift correction.
By pressing
Remove
the drift correction will be undone and the button becomes inactive again. Also
once
Apply
was pressed and a
Model-Based
drift correction was performed, the
Correction
button in
the display field, only available in the
Show all
mode, becomes automatically active, which indicates that
the image displayed is one where the drift correction has been applied.
By pressing the active
Correction
button it becomes inactive and the image will be displayed without the
drift correction applied. The drift correction is hereby not undone in the molecule table, only in the
displayed image. Hence, by repeatedly pressing the
Correction
button, you can switch the image display
repeatedly between drift corrected and not corrected.
Fig. 623
SMLM-Drift view control tab for Model-based Drift correction after a drift correction has been
performed
Drift correction is not applied to the sum widefield image (
SWF
channel), so there might be an
offset between the
SR
and
SWF
channels after drift correction.
The
Model-Based
drift correction performs the drift
correction in different segments, which can be set in the
Model-Based Drift Correction
: field in the
Segments
drop down menu and its input box (Fig. 624). The
number of frames of the
SMLM
time series within each
segment is determined by the total number of images
divided by the number of segments rounded up or down
to obtain integer numbers. Hence the last segment
might contain less, equal or more frames than the
preceding segments. The drift correction will be
performed for each segment separately and than the
drift between the segments corrected.
Fig. 624
Segments drop down menu