ELYRA 7
Hardware Aspects
ZEISS
03/2019 V_02
000000-2262-999
13
2.2
Optical Diagram of ELYRA (Schematic)
Fig. 7
Optical path, schematic (ELYRA 7)
The diagram is a schematic representation of the ELYRA system (Fig. 7).
Laser light is focused into the back focal plane of the objective to obtain widefield illumination in the
specimen. For TIRF mode dependent on the angle of the TIRF mirror either Epi-, HILO- or TIRF-illumination
is achieved.
For SIM mode only Epi-illumination is available as the TIRF mirror remains in a fixed position. Light
emitted from the specimen is directed back via a reflector cube that separates the emission from the
excitation light. The fluorescence signals are directed to individual EMCCD cameras.