ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
217
5.3.19
Processing – SIM
Licensing
Instrument
standard
optional
LSM
ELYRA
Lightsheet Z.1
Tool accepts data recorded in SIM mode on ELYRA. In data sets containing other
types of image data as well ("mixed mode" files), this tool selectively processes the
SIM data. "Experimental PSF" (requires Z-stacks of point-like objects) and "Fourier
Transform" accepts input other than SIM data as well. See full description of tool set
for details.
The
Processing
SIM
tool
works exclusively for data
acquired in the
SIM Apotome
as well as
Stripe
&
Lattice
SIM imaging
modes.
The
SIM
function allows automatic or manual
processing of a set of structured images (lattice SIM or
Sripe SIM obtained by phase shift (Lattice SIM) or by
phase shift and rotation of the grid (Stripe SIM).
SIM
processing tool can use SIM Apotome as well as
Lattice SIM and Stripe SIM data.
Select the the
SIM
tab to select the
SIM
Processing
tool (Fig. 370). Activate the image you want to
process in the image container and press the
Select
button to load the image. Then expand the
Method
Parameters
tool window to have access to setting
parameters (Fig. 371).
Fig. 370
Processing – SIM
Fig. 371
Processing – SIM: Methods
Parameter