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ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
142
000000-2262-999
03/2019 V_02
TriggerIN 1 and stopping it via TriggerIN 2. Alternating between the two trigger inputs in essence
pauses the time series for a desired time.
5.2.21
Multidimensional Acquisition –
Positions
When the
Positions
tool tab is expanded the
Positions
tool becomes available (Fig. 230).
This function comprises two main applications.
First, it is used to take images of a specimen at
freely defined positions (
Positions List
mode).
Second, it is used to image specimens, typically
cells, which are grown in sample carriers like plates
with a set number of wells (
Sample Carrier
mode).
5.2.21.1
Position List
Press the
Position List
button to obtain the
Position List
control panel (Fig. 231).
Clicking the
Add
button adds the current position
and its coordinates to the list of positions.
Displayed are the number of the positions, their
x
(
μ
m)
,
y (
μ
m)
and
z (
μ
m)
coordinates and also
their
offset
values (µm). See the description of
Setting individual offset values for positions
in Position List mode
(below) and also the tool
description of
Focus Devices and Strategy
for
details on offset values (section
and Strategies for Widefield Imaging Modes
).
Highlighted positions can be removed by pressing
the
Remove
button. Alternatively the whole list
can be deleted by pressing the
Remove All
button.
It is also possible to move the stage to the highlighted position by clicking the button
Move to
. The
order of positions in the list can be modified by pressing the buttons
Up
or
Down
.
You can save and load pre-saved positions by pressing the
Save…
and
Load…
buttons, respectively,
which will open Windows Explorer.
The list of positions is consistent with the positions defined in the
Stage
tool.
Note: When the list of positions is deleted in the
Position list
, it is also deleted in the
Stage
tool.
This is not the case the other way round.
It is recommended to save the position list as relative positions. The format "Positons list files
(relative)" should be selected in the Windows Explorer. This format allows to reuse the positions for
other experiments later on. Just make sure that the reference position for the list (for example the
position 0) matches with the actual zero position in your sample. Start with positioning the sample
at the desired zero position and set the stage to zero before loading the position list.
Fig. 230
Positions tool
Fig. 231
Position List control panel