ELYRA 7
Center Screen Area / Image Containers - Display and Image Analysis
ZEISS
03/2019 V_02
000000-2262-999
379
You can select between:
−
Automatic
(Fig. 625): The algorithm determines the optimal number of segments, but will not
exceed the maximum (
max
) number as set in the
max
input box. Up to 50 segments can be
selected.
−
Selected
(Fig. 626): The algorithm takes exactly the number of segments set in the input box. Up
to 50 segments can be selected.
−
Adapted
(Fig. 627): This mode can (but must not) be used when the SMLM time series was
interrupted by automatic z-focus drift control. Then the algorithm will take the frames between
z-focus drift controls as segments. The input box is inactive and cannot be edited. It displays the
number of segments of the SMLM series that will be used dependent on the number of z-drift
controls; the number of segments equals number of z-drift controls plus 1. If no z-drift correction
was performed, the number "1" is displayed, which than will be used as the number of segments.
Fig. 625
Segment drop down menu with Automatic selected
Fig. 626
Segment drop down menu with Segments selected
Fig. 627
Segment drop down menu with adapted selected
The
Display
field with the
Drift Diagram
,
Drift table
and
Correction
is only available in the
Show all
mode (Fig. 628).
Fig. 628
Display field
The number of optimal segments depends on the drift. If it is constant throughout the experiment,
lower numbers of segments will give more robust fits. In contrast, if drift is changing for example
direction during the experiment, more segments will be beneficial. If your drift curves are straight
lines, you may reduce the number of segments. However, if kinks are visible, it can be beneficial to
increase their numbers. If the number of kinks increases with increasing segments, you should stay
with more segments, otherwise return to the number of segments from which the number of kinks
does not increase any more.