ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
274
000000-2262-999
03/2019 V_02
−
EPI
illumination using 100 nm bead sample. In this case, focus on the beads and move slider until
beads get obscured in the diagonal from left upper to right lower corner of the image. This will
indicate, that the slider obscures the field of view and will be in the middle (Fig. 463).
−
EPI
illumination using 100 nm beads sample and using Bertrand lens to look into the back aperture
og the objective. Use Tube lens 1x . Move the slider until it divides the puplil circle intio two equal
halves (Fig. 464).
Fig. 463
Adjusting the 3D-PALM slider in Epi using obscuring of
fluorescence in the diagonal of the image with non-optimal
(left panel) and correct (right panel) positions.
Fig. 464
Adjusting the 3D-PALM slider in Epi using imaging of the
back focal plane with non-optimal (left panel) and correct
(right panel) positions.