ELYRA 7
Level-adjustable Sample Holder with Insert for Piezo Stage
ZEISS
03/2019 V_02
000000-2262-999
15
Fig. 5
Level-adjustable Piezo stage insert – Dimensions of the sample holder
The sample holder is designed to mount 36 mm Petri dishes, standard slides (76 mm x 26 mm) and
Labtek
TM
chambers (Fig. 5). Slides can be clamped with the lower spring (Fig. 4,
ls
); dishes and Labtek
TM
chambers with the upper spring (Fig. 4,
us
). To secure a sample loosen the spring by pulling up, position
the sample and clamp it by pressing the spring down until a resistance is experienced. To losen the
sample, simply lift the spring.
It is recommended not to place the lower clamp in a position directly over the objective lens to
avoid back reflection of the excitation light. Rather clamp the sample at position offset from the
lens position. Also one should restrain from using the lower spring for clamping dishes or
Labtek
TM
chambers, as the induced strong strain on the spring can result in its damage. If not in
use the springs can easily be stored by bringing them in a parallel position to the sample holder’s
longer edge. Secure in this position by pressing the spring down.
The three-point mounting allows for convenient leveling of the stage. Before leveling a sample one
should make sure that the stage insert is correctly pre-adjusted. The cleft width measured at the corner of
screw
sz
should be around 2 mm in size and is pre-set as such by the factory (Fig. 4).
If it happens to be substantially off, i.e. more than 1mm, one can adjust the distance with screw
sz
back
to 2 mm. To pre-adjust the stage insert the cleft width viewed from the right (Fig. 4,
csv
) and back sides
cbv
) should be constant over their whole length; in other words the edges of the base plate and
sample holder should be parallel. If this is not the case screws
sy
and
sx
(Fig. 4) can be used to adjust the
edges to be parallel.
When the cleft widths are constant and 2 mm in extension, there is enough range to adjust the
leveling of the sample (about 1
o
tilt possible in both directions). If the cleft space is substantially
larger or smaller and / or the edges are inclined to each other the range might be not sufficient
for proper leveling.