ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
229
In either case, the other loaded image does not play a role. In the
coefficient table
the channel (
Ch
)
assignment column is replaced by an identity (
Id
) column that numbers the channels in their ascending
order starting with 0.
The assignment table represents the selected image,
either
Input image
(
Input 2
check box inactive) or
Input image 2
(
Input 2
check box ticked).
Note that the dimensionality must not be the same as in
the
coefficient
table. The
assignment table
lists the
channels (
Ch
) and the laser lines used (
Laser
). With the
identity (
ID
) drop down menu (Fig. 403), an ID from the
coefficient table
can be assigned individually to a
channel. That channel will then be corrected exactly with
the coefficients of that
ID
.
If no alignment should be performed
do not align
can
be chosen.
The
channel alignment
tool is generically programmed to have high flexibility. Care should
therefore be exerted in the control which channels are how aligned as there are no warnings, if
unrelated channels are aligned to each other.
If you draw Regions of Interests (ROIs) in the image, they will be taken for alignemnt. For parabolic fit,
you should have at least 3 well spaced regions. If you have fiducial markers, you can use them by setting
the ROIs around them. Please be aware that in the z-direction the algorithm scans the whole z-Stack and
takes the brightest area in th z-direction. If your beads are not the brightest structure, than the alignment
will fail.
Fig. 403
ID drop down menu