ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
176
000000-2262-999
03/2019 V_02
5.3.9.2
Ratiometric Dyes
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Fura-2, Indo-, SNARF, Cameleon, Ratiometric Pericam, Phluorin.
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Display fluorescence ratio R over time
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Display fluorescence ratio R corrected for background/autofluorescence over time
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Calculate absolute ion concentrations (pixel by pixel) via titration calibration (known ion concentrations
applied to the cells – in situ – or in solutions – in vitro or equation calibration where possible [Fura-2,
Indo-, SNARF]
•
Calculation of R eliminates artifacts and uncertainties caused by
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inhomogeneous dye distribution
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photobleaching
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may be applied with moving cells.
Ratiometric Dyes - Online Ratio
R(t1) = F1(t1) / F2(t1), R(t2) = F1(t2) / F2(t2) .
Fig. 280
Ratiometric Dyes – online ratio