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ELYRA 7
Center Screen Area / Image Containers - Display and Image Analysis
ZEISS
03/2019 V_02
000000-2262-999
371
6.18.4.1
Fiducial Based Drift Correction
SMLM-Drift Fiducials
selection is used to perform a drift correction based on fiducials like gold particles
(Fig. 606).
What is to be considered to be a fiducial can be defined in the
Find Fiducials:
section, where the
following parameters can be set:
−
Min On Time (%)
: Sets the minimum on time in percentage of the whole time series an object has
to be detected in order to be regarded as a bona fide fiducial. 75% is the default value and means
that the object has to be found in at least 75% of the recorded frames.
−
Off Gap (frames)
: Sets the maximum number of frames an object can be off consecutively in
order to be still regarded as a fiducial.
−
Capture radius (pixel)
: Sets the maximum radius around an object, where another object is
tolerated in order that the object is regarded as a fiducial.
When
Apply
is pressed, the software identifies on behalf of these criteria potential fiducials and uses
them automatically to correct for their drift. The correction vector calculated from the average of all
selected fiducials will then be applied to the SMLM image (
SR
channels).
Once
Apply
is pressed, the
Remove All
button becomes available, when fiducials have been identified
and a drift correction has been performed (Fig. 607). Hence an inactive
Remove All
button is indicative
of a SMLM image without drift correction. By pressing
Remove All
the drift correction will be undone
and the button becomes inactive again.
Drift correction is not applied to the sum widefield image (
SWF
channel), so there might be an
offset between the
SR
and
SWF
channels after drift corection.
Fig. 606
SMLM-Drift view control tab of the SMLM view tab