ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
255
5.3.23.3
ICS Correlation
•
Activate
ICS correlation
in the
ICS
tool. The
ICS correlation
menu will appear (Fig. 431).
•
Highlight the image in the image container and
press the
Select
button. This will be your
Input
image.
•
This function is only available in the LSM
imaging mode
A correlation image will be computed based on the
settings and displayed in the
Preview
window. As
an output image an image with the selected
average subtracted is computed and the result
presented in the
Preview
window.
•
The correlation to be computed can be selected
from the
Correlation
drop down menu
(Fig. 432). For one channel recordings, only
Spatial auto-correlation (S+A)
is available, for
a two channel recording in addition
Spatial
cross-correlation (S+C)
.
The way of average subtraction can be selected
from the
Remove structures
drop down menu
•
You can select between
None
(no average
subtraction),
Slowly moving structures
(subtraction of moving average, the frame size
of which can be selected in the
Average
frames
input box),
Immobile structures
(+Stack)
and
Immobile structures (+Plane)
(subtraction of total average and adding
average scalar of all pixels from one frame to
each corresponding frame).
Fig. 431
Processing – ICS correlation
Fig. 432
Correlation drop down menu
Fig. 433
Remove structures drop down menu