OPTi CCA-TS2, Руководство оператора, Страница: 259 / 299

Operator’s Manual – OPTI CCA-TS2 Lac-3
ANALYTES LACTATE
Chemical Test level
Concentration
d max Lactate Level
mM
Interference
Intralipid 1% 0.2mM
2 NO
5 NO
Methylene Blue 0.125 mM 0.2mM
2 0.6 mM
5 1.1 mM
Sodium Chloride 20 mM 0.2mM
2 NO
5 NO
Reproducibility
Controls
Within run precision (S
wr
) and Total Precision (S
T
), were determined from 2 runs per day with 2 replicates
per run over a period of 20 days following the CLSI guideline EP5-A2. Typical results for 3 different
control levels are shown below:
Lactate (mmol/L) OPTI Check Level 1 OPTI Check Level 2 OPTI Check Level 3
Days Run 20 20 20
Total Average 1.02 2.50 4.59
Within Run St. Dev. (S
wr
) 0.06 0.11 0.18
Within Run % CV 5.6% 4.2% 4.0%
Total Precision St. Dev. (S
T
) 0.07 0.11 0.22
Total % CV 6.4% 4.5% 4.8%
Whole Blood
Within-run precision in whole blood samples was evaluated at 3 different concentrations on the OPTI
CCA-TS2. One repeat was run on 10 different analyzers in parallel due to the rapid change of lactate in
whole blood. The table below shows the Averages, the Standard Deviations and the %CV calculated from
10 samples for each level.
Lactate (mmol/L) Whole Blood
Level 1 Level 2 Level 3
Average 3.85 1.13 6.17
St. Dev 0.14 0.05 0.20
%CV 3.76% 4.42% 3.16%
n 10 10 10