Cl-2
Operator’s Manual – OPTI CCA-TS2
CHLORIDE
ANALYTES
Interferences
The OPTI CCA Cl
-
sensor does exhibit a significant (greater than 2:1) positive interference from bromide,
iodide, interlipid and nitrite. Minor interference is observed from phenylacetic acid salicyate and
thiocynate. This testing was done on the OPTI CCA platform, with a standard OPTI style Chloride sensor.
No changes were made to the sensor or the measurement principle, so the interference of the OPTI CCA-
TS2 is equivalent to the OPTI CCA.
Heparin salts are the only acceptable anticoagulants. Other anticoagulants such as citrate, EDTA, oxalate,
and fluoride cause significant interferences to the electrolyte sensors.
Only clear, uncolored quality control materials, such as OPTI CHECK or OPTI CHECK PLUS brand
aqueous controls should be used with the OPTI CCA-TS2 system. Colored materials, including
proficiency testing materials, may interfere with the chloride measurement, or fail to be properly
aspirated
.
Reproducibility
Controls
Within run precision (S
wr
) and Total Precision (S
T
), were determined from 2 runs per day with 2 replicates
per run over a period of 10 days following the CLSI guideline EP5-A2. Typical results for 3 different
control levels are shown below.
Cl
-
(mmol/L)
OPTI Check Level 1
OPTI Check Level 2
OPTI Check Level 3
Days Run
10
10
10
Total Average
95.3
107.1
115.6
Within Run St. Dev. (S
wr
)
0.6
1.4
0.5
Within Run % CV
0.7%
1.3%
0.4%
Total Precision St. Dev. (S
T
)
0.7
1.4
0.6
Total % CV
0.8%
1.3%
0.5%
Whole Blood
Within-run precision in whole blood samples was evaluated at 3 different concentrations on the OPTI
CCA-TS2. The table below shows the Averages, Standard Deviations and % CV calculated from 10
samples for each level.
Cl
-
(mmol/L)
Whole Blood
Level 1
Level 2
Level 3
Average
104.1
141.3
57.5
St. Dev
0.3
1.0
0.3
%CV
0.28%
0.73%
0.51%
n
10
10
10
Содержание CCA-TS2
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