Thermo Scientific Phadia 100, User Manual

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Reagent and Sample Handling Phadia 100 - User Manual
P a g e 3 . 4 12-3501-20/12
Recommendations regarding the
performance
Since high levels of IgG, IgA and IgM are common in many sources that can be
found in the surrounding, like in sputum, human skin flakes etc, a correct
handling of ImmunoCAP IgA, ImmunoCAP IgG, ImmunoCAP IgG4, EliA IgA,
EliA IgG and EliA IgM reagents becomes very important to avoid contamination
and to get reliable results. Some general points to be aware of, when handling
reagents, can be found in this chapter.
ImmunoCAP Specific IgG, ImmunoCAP Specific IgG4, EliA IgA, EliA IgG and
EliA IgM conjugates, calibrators and curve controls are very sensitive to
contamination from IgA, IgG, IgM and IgG4 in patient samples, due to the large
amount of IgA, IgG, IgM and IgG4 in human sera. We know from our
investigations that only 0.01 µl of a serum sample added to the conjugate vial
is enough to make an increase of the response level, which will lead to a non-
accepted calibration curve.
It is also very important that the instrument is well maintained according to the
maintenance procedures in Chapter 11 in Phadia 100 User Manual.
Things to be aware of before and after an assay
• Perform a Daily Rinse before each assay.
• Use the Consumption list to make sure that there are enough volumes of
reagents loaded. Always make sure that there is
one liter
of both Washing
Solution, prepared according to the DfU, and Rinse Solution added to the
bottles.
• Check tubing connections to the Washing Solution and Rinse Solution
bottles. The O-rings shall be lubricated regularly according to the
Monthly Maintenance. The screw caps of the bottles should be properly
tightened before the tubings are connected, this will secure that the
tubings are not twisted.
• In general it is not recommended to pool reagents. Conjugates can be
pooled
once
, but only with another conjugate having the same lot
number and only from a new vial to an old vial. Make sure to use the vial
with the pooled material only
once
and to discard the vial after performed
assay.
• Do not use the conjugate vial for more than four assay runs per vial.
Reagent Handling, ImmunoCAP Specific IgA,
ImmunoCAP Specific IgG, ImmunoCAP
Specific IgG4, EliA IgA, EliA IgG and EliA
IgM