Thermo Scientific Phadia 100, User Manual

Page: 73 / 357

Routine Assay Run Phadia 100 - User Manual
P a g e 2 . 4 2 12-3501-20/12
1.Prepare Run
3.Load and Start?
Load and Start
These steps will include the loading of system liquids, reagents, samples,
ImmunoCAP/EliA Wells and finally starting up the run. There will be an
amount and a position given for each component. In some cases you have to
enter the calibration code of the conjugate and ImmunoCAP/EliA Wells. Each
step is confirmed with
enter
.
The dead volume varies between tubes, please see chapter 3.
For all the other reagents (excluding calibrators, controls and CC) the dead
volume is already included in the calculated volume.
If you use the barcode reader, you have to follow the loading order as described
below. The instrument will recognize the component when reading the barcode.
If you are loading the components manually, please be sure to load the correct
component in the correct place. The instrument will not warn you if the
component is not the correct one.
NOTE! Check for air bubbles or film on the surface of the reagents/samples. If
there are air bubbles or film, pinch them away. Otherwise the pipette sensor will
not detect the surface properly.
Good laboratory practice dictates that every precaution must be taken to avoid
evaporation and contamination. Therefore it is recommended to remove and
recap the QC vials from the instrument as soon as the pipetting of samples are
finished. For EliA the QC vials from Phadia AB are single use and should be left
in the instrument during the run.
When the run is processed the result will automatically be printed.
1.
In Prepare Run mode
press
yes
.
2.
In Enter Request mode
press
3
.
3.
Press
yes
.
Phadia 100 Ver.3.0 14:10:17
1.Prepare Run?
1.Prepare Run
1.Enter Request?
NOTE! Make sure that the volume of the sample
in each tube is sufficient for the required amount
to be withdrawn. The dead volume of the sample
tubes must be added to the sample volumes
specified on the Consumption List.