Thermo Scientific Phadia 100, User Manual

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Phadia 100 - User Manual Routine Assay Run
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Load and Start
Select 1.3.Load and Start ; the software will guide you through loading of
reagents and samples. Be sure to load the correct reagent/sample in the correct
place in the sample carousel. Inspect the surface of all reagents/samples for air
bubbles or film. In case of air bubbles or film, pop them away with a clean pin.
Be extremely careful not to cross-contaminate reagents.
Dead volumes of reagents are included in the volume asked for by the software.
Dead volumes of samples are, however, not included. This is because dead
volumes vary a lot between sample tubes. For example of dead volumes of
sample tubes, please see Chapter3, Reagent and Sample Handling. Be sure to
load enough sample.
Check the volume of the Washing and Rinse Solution. One liter is needed for
each solution. The prepared Washing Solution is stable for one week when
stored in room temperature. Check also that the tube connections are firmly
pressed down into the bottles. Press down until there is a clicking sound.
When loading ImmunoCAP/EliA Well, be sure to load the correct allergen/
antibody.
Select 1.3.4.Start Process . The instrument will start the process as soon as the
right temperature level is reached (37ºC ± 0.5ºC). The process takes about 2.5
hours and a laboratory report will automatically be printed when the process
is ended.
Maintenance
Maintenance has to be done in order to keep the instrument in good condition
and secure the instrument performance. Please read chapter 11, Maintenance.
WARNING! Washing Solution may cause
sensitization by skin contact. Wear protective
gloves!