Thermo Scientific Phadia 100, User Manual

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Phadia 100 - User Manual Error Handling
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20 20
20 20
20 Empty instrument manually and run Monthly Maintenance
Unload Sample Carousel, open Processing lid and lift up ImmunoCAP holder
disc. Throw away ImmunoCAP or EliA Wells. Check that no ImmunoCAP or
EliA Wells are left in or under the Elution Wells Disc. Check also under Wash
Rake. After emptying the instrument, perform a complete Monthly Mainte-
nance, see Chapter 11.
21 21 21 21 21 Check if Wash Sol/Rinse have been mixed
Shake gently both Washing and Rinse Solution bottles, the bottle with Washing
Solution will foam.
22 22 22 22 22 Check Lot Specific Code for calibrator EliA wells
Check on Laboratory Report that right Lot Specific Code has been used. Lot
Specific Code is written for every EliA Well position.
23 23
23 23
23 Load old run and rerun Samples
Check that sample and reagent volumes are enough for the rerun. Look at
consumption list and include dead volume for the sample tubes. Rerun the
results that have not been reported. Load the assay run again by first using
1.5.Load Old Run.
24 24 24 24 24 Rerun the results that have not been reported
Check that sample volume is enough including the sample tube dead volume,
otherwise new patient sample is needed. Use
1.1.Enter requests
and put in the
requests that have not been reported.
25 25
25 25
25 Replace the Washing Solution and the Rinse Solu-
tion before next run
Clean the bottles thoroughly before preparing new fresh solutions.
26 26
26 26 26 Ensure that Wash Sol/Rinse are properly prepared
The Washing Solution Concentration is of big importance for several tests.
Always prepare according to Directions for Use. Always use purified water
when preparing Washing Solution and Rinse Solution.
27 27 27 27 27 Rerun with new fresh Conjugate vial
Rerun the results that have not been reported. Load the assay run again by first
using
1.5.Load Old Run.
If only part of the assay run is not approved, patient
requests need to put in once again 1.1.Enter Requests . Avoid contamination of
conjugate vial with sample. See Chapter 3, Reagent and Sample Handling.