Thermo Scientific Phadia 100, User Manual

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Error Handling Phadia 100 - User Manual
P a g e 1 2 . 1 2 12-3501-20/12
NORMAL VALUES
For ImmunoCAP between 10 - 20 RU and for EliA between 15 - 35 RU.
Corrective actions:
Follow instructions in Action List in order to identify or eliminate the errors.
Clean and dry (not with Paper tissue) Elution Wells Disc, see Chapter 11,
Cleaning of the process chamber.
Avoid mixing up the Development Solution vial cap and the Conjugate vial cap.
Small amounts of Conjugate coming in contact with the Development Solution
affects the background signal of the Development Solution. Residues of
conjugate on the vial cap is enough to affect the Development Solution function
next time it will be used.
Perform Monthly Maintenance as corrective action before next run in order to
clean the instrument. Monthly Maintenance should be done even if the regular
Monthly Maintenance has been done recently. Something has happened during
the run and the instrument needs to be cleaned, like after Rinse Solution and
Washing Solution distribution problems, lack of maintenance or vacuum
problems. After going trough all other actions on the list first and thereby
eliminate the error it could be that Monthly Maintenance can be skipped this
time.
If the error remains contact your local Phadia representative.
THE DIFFERENCE BETWEEN REAGENT BLANK
AND RINSE BLANK IS TOO LOW
The difference between the Reagent Blank and Rinse Blank is less than 1.875 RU
which is too low.
What can cause the problem:
Broken cuvette. Rinse Solution or Washing Solution in both bottles. Rinse
Solution that is contaminated.
Corrective actions:
Follow instructions in Action List in order to identify or eliminate the errors.
Check visually if the Cuvette is broken. Is the Cuvette broken, clean up the
Processing Chamber. Make sure there is no glass in the process chamber, check
Elution Wells Disc, lift up and look underneath also. Look for misplaced
ImmunoCAP/EliA Well on Elution Wells Disc, under Elution Wells Disc and
under Wash Rake. If ImmunoCAP/EliA Well is found, that is probably the
cause.
If ImmunoCAP not dispensed and Check ImmunoCAP Dispense have occurred
prior to this error situation, that can be an indication of future problems when
there has been ImmunoCAP Dispense errors. Always check that no misplaced
ImmunoCAP/EliA Well is in the Processing Chamber after this error message.
Always empty the ImmunoCAP Waste Container after every run.