Processing Parameters
Blank Read Times
User Defined Reagents
UniCel DxC Systems Instructions For Use A13914
Page 9-14
October 2005
Blank Read Times
Blank read times indicate when the blank absorbance is to be read.
• Time -180 to -1 is a reagent blank.
• Time 1 is when sample is added to the cuvette.
• Time 1 to 720 is a sample blank.
• Time is entered in 1-second intervals, but readings will begin and end at the closest
8-second interval.
• Read times should not coincide with a pipetting cycle.
Initial Read Times
The initial read time is defined in the initial read time window for the rate or end point
measurement. The end time must be at least 8 seconds greater than the start time.
If the initial read times are not entered, the default values are defined as follows:
• Reaction starts with sample addition (sample is added at cycle 1). Initial read starts
at cycle 1 and ends at cycle 2.
• Reaction starts with trigger reagent (trigger reagent is added at cycle N). The initial
read starts at cycle N and ends at cycle N + 1.
Reaction Read Times
Reaction read times indicate when the reaction is to be read.
• Time 1 is when sample has been added to the cuvette.
• Time is entered in 1-second intervals, but readings begin at the next 8-second
interval.
Table 9.23
Blank Read Times
Allowable Entry
Start Read
-180 to 1704 seconds, except 0
End Read
-180 to 1720 seconds, except 0
Table 9.24
Initial Read Times
Allowable Entry
Start Read
1 to 1704 seconds
End Read
9 to 1720 seconds
