Multifors 2 - Parallel Bench-top Bioreactor
Technical Data
28 March 2018
Page
37
of
211
3.5.7 Gassing Cell Culture System
Basically, the entire gas entry takes place via the sparger.
Additionally, head space gassing with air is possible, too. This pre-
vents from accumulation of CO
2
in the head room and reduces
foam.
Optionally, CO
2
gassing can be used for pH control instead of liq-
uid acid. Gas entry also takes place via sparger.
The specific gassing rate, calculated for the max. working volume
is for each vessel size and each variant 0.1 min
-1
.
For head space gassing, this rate is 1 min
-1
for each vessel
size
and variant.
Variant Standard
Gas(es)
Gas flow
control
Accuracy
MFC
Gas mix control
Air
1 MFC
± 0.3 %
(final value)
± 0.5 %
(measured
value)
----
Air + O
2
1 MFC
2 solenoid valves
Air + N
2
1 MFC
2 solenoid valves
Air + O
2
+ N
2
1 MFC
3 solenoid valves
Variant High End
Gas
Gas flow control
Accuracy MFC
Air
1 MFC
± 0.3 %
(final value)
± 0.5 %
(measured value)
Gases
Gas flow control and gas
mix control
Air + O
2
2 MFC
Air + N
2
2 MFC
Air + O
2
+ N
2
3 MFC
Head space gassing (Air) & CO
2
sparger gassing (pH control)
Gas
Gas flow con-
trol
Accuracy
Rotameter / MFC
Gas mix control
Air
Rotameter
Rotameter
± 4 %
----
CO
2
± 4 %
1 solenoid valve
1 MFC
± 1 %
1 MFC
1
1
)
same MFC (mass flow controller) as MFC for gas flow
