Multifors 2 - Parallel Bench-top Bioreactor
Operation
Page
160
of
211
28 March 2018
Escape of noxious gases
WARNING!
Explosion and/or danger of suffocation due to
escape of dangerous gasses!
Leaky or incorrectly fastened hoses on the gas
supply (particularly regarding the supply of pure
gases such as oxygen or nitrogen!), both from the
supply to the equipment and subsequently to the
vessel, can lead to the escape of dangerous gases.
This can be a danger of gas explosion and/or dan-
ger of suffocation as well as a hazard for the health
of the operator.
Therefore:
– Check that all supply hoses are intact.
– Check that all supply hoses are correctly fas-
tened and secured.
– Check that supply hoses are not bent/kinked.
– Always check the pressure control of the gas
supply is correct when the supply is opened.
– Always check the gas supply is closed before
removing the gas supply hose from the base
unit to the vessel.
– Always close the gas supply if the equipment is
not in use.
– Always obey all local regulations concerning the
handling dangerous gases.
Dangerous exit gases
WARNING!
Danger of explosion and or asphyxiation other
health dangers to the user due to dangerous
exit gases!
Dangerous gases can be generated when cultivat-
ing micro-organisms, which get into the ambient air
via the exit gas cooler. The following dangers exist:
– Danger of explosion and/or asphyxiation due to
exhaustion of oxygen or hydrogen.
– Danger of asphyxiation due to exhaustion of ox-
ygen free gases e.g. carbon dioxide
– Danger of asphyxiation and other health risks
such as dizziness and mental confusion due to
exhaustion of carbon dioxide.
Therefore:
– Ensure the exit gas is safely deviated
– Ensure the exit gas filter is intact.
– Ensure the laboratory/working site has a suffi-
cient aeration.