Multifors 2 - Parallel Bench-top Bioreactor
Table of Contents
Page
6
of
211
28 March 2018
7.2.2.2
Mounting the pH and pO
2
Sensors ...................................... 121
7.2.2.3
Mounting the Antifoam Sensor ... 125
7.2.3
Preparing the Reagent Bottles, Hoses and
Pumps .........................................................128
7.2.4
Calibrating the Pumps ................................128
7.2.5
Connecting the Reagent Bottles to the Pumps
and Vessels ................................................129
7.2.6
Preparing the Sampling System Super Safe
Sampler ......................................................132
7.2.7
Mounting the Exit Gas Cooler .....................136
7.2.8
Preparing the Port in the Vessel Top Plate for
Inoculation ..................................................137
7.2.9
Preparing the Sparger ................................139
7.2.10
Attaching the Pump Heads to the Vessel
Holder and Support Frame .........................141
7.2.11
Transport to the Autoclave .........................142
7.3
Sterilisation in the Autoclave ......................................143
7.3.1
Sterilisation Methods ..................................143
7.4
Preparations after Sterilisation ...................................146
7.4.1
Mounting the Vessel Holder with the Vessels
on the Basic Unit.........................................146
7.4.2
Filling the reagent Hoses ............................149
7.4.3
Connecting the Sparger to the Gas Supply 150
7.4.4
Connecting the Temperature Sensor .........151
7.4.5
Testing the Antifoam Pump, connecting the
Antifoam Sensor .........................................152
7.4.6
Connecting the pH and pO2 Sensors .........154
7.4.7
Polarising a (polarographic) METTLER pO
2
Sensor ........................................................156
7.4.8
Calibrating the pO
2
Sensor .........................156
7.4.9
Connecting the Exit Gas Cooler .................157
8
Operation ............................................................................159
8.1
Safety .........................................................................159
8.2
Starting Fermentation.................................................162
8.2.1
Switching on the Equipment .......................163
8.2.2
Inoculation ..................................................164
8.2.3
Sampling .....................................................166
8.2.4
Sampling with Sampling System Super Safe
Sampler ......................................................166
8.3
Ending Fermentation ..................................................170
8.3.1
Harvest .......................................................171
8.3.2
Shutting down the System, switching off the
Equipment ...................................................173
