Multifors 2 - Parallel Bench-top Bioreactor
Preparations for Use
28 March 2018
Page
149
of
211
7.4.2 Filling the reagent Hoses
To prepare the reagent hoses for operation, they must be filled with
reagent. Proceed as follows:
Procedure
1.
Switch the equipment on at the main switch.
2.
Open the clamps on the corrective reagent tubes.
3.
Fill the reagent hoses individually, successively and manually.
Observe the following points:
Provided that the reagent bottles are correctly connected:
Press the rocker switch to the right side
the pump runs forward (counter clockwise), reagent is
sucked in from the reagent bottle and is pumped in the
direction of the vessel
Press the rocker switch to the left side:
the pump runs backwards (clockwise), reagent is liquid
is sucked in from the culture vessel and is pumped in
the direction of the reagent bottle
Release the rocker switch just before liquid visibly enters the
addition port adapters, respectively the dosing needle of the
antifoam sensor on the vessel top plate.
NOTICE!
It is acceptable and time-saving to fill the reagent
tubes automatically and simultaneously when oper-
ating several units (parallel bioreactor system) For
details see the separate manual for the touch
screen software.
