Multifors 2 - Parallel Bench-top Bioreactor
Setup and Function
28 March 2018
Page
83
of
211
NOTICE!
Disinfection of the automatic sample valve by wip-
ing with a commercial disinfectant can be accom-
plished before fitting the syringe, in order to guar-
antee the sterility of the sample. This measure is
not necessary to ensure the sterility of the bulk cul-
ture and can be omitted.
Following sampling, a second syringe can be fitted and air pushed
in via the sterile filter, in order to displace culture solution from the
sample hose and the dip tube of the vessel. With a conventional
sampling system, the next sample cannot be taken immediately, as
rinsing of the sampling hose and the immersion tube is necessary.
By previously removing most of the culture in the sampling line,
this sampling system can save culture volume, which is particularly
important with small vessels and/or frequent sampling.
The dead volume of the culture remaining in the group of valves
after flushing with sterile air amounts to a few µl and is negligibly
small, therefore, in relation to the usual sample volume of 10 mL.
If the withdrawal of a very small sample volume is required, with
minimum possibility of falsification, a small quantity of culture solu-
tion (e.g. 1 mL) can be introduced and rejected before the actual
sample is taken
Designated use
The Super Safe Sampler can be employed for aseptic sampling for
the following applications:
Cell cultivation
Bacterial cultivation
Fungal cultivation, if formation of mycelia which might clog the
valves does not occur.
Algal cultivation, if formation of cell aggregates which might
clog the valves does not occur.
The Super Safe Sampler is autoclavable and for this reason reusa-
ble. It can be used in combination with practically any pressureless
culture vessel like:
Autoclavable stirred tank reactors
Reusable spinner flasks
Disposable sterile spinner flasks
Disposable sterile culture bags
