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High Technology, Inc.
109 Production Road,
Walpole MA USA 02081
Document Number
OM-E-FC-360-30
Revision Level
Rev. 3
Prepared By
J.S. Bolio
Approved By
S. Titov
Effective date
8/15/2016
Operator’s Manual: HTI BioChem FC-360
Page 172 of 217
HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL
When the ISE Module is not operating properly, approach troubleshooting as a logical sequence of events.
Isolate the problem area to avoid unnecessary component replacement and down time.
Troubleshooting can be categorized into three main areas. These areas are:
Fluid delivery, electrode stability, and communication.
Once your chemistry system is developed to a point that the communication is stable and the data
transmission is properly interpreted, troubleshooting should focus primarily on fluid delivery and electrode
stability. As these are related, sometimes the same symptoms can have different causes. Most problems
can be corrected while the ISE Module is still installed in your chemistry system.
Fluid Delivery
It is necessary to perform a Pump Calibration cycle each day. This cycle will calibrate the pumps that
dispense Cal A and Cal B, and position fluid in front of the electrodes. The waste pump moves solution
from the Sample Entry port to the electrode area for measurement. As the tubing ages and samples are
passed through the system, the positioning of the solution will change. The pump uses a stepper motor
that counts how many steps it takes to move the solution to the correct position.
By calibrating the pump each day, the ISE Module can now calculate the relationship between volume and
number of pump steps. This will enable the pumps to dispense an accurate volume of calibrant, and for the
solution to be accurately placed in the proper location for analysis.
Problems can be caused by a partial obstruction from a clot in the tubing from the exit tube to the waste
pump, a sharp bend in the waste tubing that restricts the flow, a misalignment of the electrodes, or from
too great a length of tubing from the exit tube to the waste pump. As the pump tries to pull the fluid from
the sample entry port into the electrodes, a vacuum develops because of the restriction. The bubble
detector detects the trailing edge of the solution (sample, calibrant, etc.) and stops the waste pump so that
the solution is in front of the electrodes. The vacuum will cause the solution to travel after the pump stops.
One of the first indications of a flow problem will be the lithium electrode response.
If, however, the solution slowly moves out of the electrodes when the pump has not been activated, there
is either a leak between the electrodes, or along the flow path. This can be tested by placing solution into
the sample entry port by hand and watch to see if the fluid level changes. In either case, the symptoms
would be similar
—the lithium or sodium millivolts would experience noise errors and the bubble detector
would trigger an error.
Electrode Stability
Errors associated with electrode instability typically include drift, noise, and slope failures. While these
errors may be caused by a failure of a particular electrode, it is necessary to explore other causes as well.
Proper operation on a daily basis is the key to keeping the electrodes stable and the system working
properly. Each day, it is necessary to perform a Cleaning Cycle. The cleaning solution removes protein
build-up in the flow paths of both the electrodes and the tubing. In high sample volume instances, it may
be necessary to perform this cycle more than once in a single day.