High Technology, Inc.
109 Production Road,
Walpole MA 02081 USA
Document Number
OM-E-FC-360-30
Revision Level
Rev. 3
Prepared by
J.S. Bolio
Approved by
S. Titov
Effective Date
8/15/2016
Operator’s Manual: HTI BioChem FC-360
HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL
Page 135 of 222
Figure 12-31
Figure 12-32
Photometric Linearity
Photometric linearity
means the photometric capacity to make absorbance readings proportional to
concentration changes for solutions of increasing concentrations of a substance which follows Beer’s law.
Required materials:
Potassium Dichromate solutions in Perchloric Acid 0.001 N of increasing concentrations and known and
certified absorbance measured using NIST Reference Standards Certificates (National Institute of
Standards and Technology). Example: 25, 50, 100 and 200 mg/l (solutions A, B, C and D respectively).
Automatic Pipette.
Procedure:
1. Dispense manually 300 µl of solution A in cuvette 5 or in any cuvette, indicating it on screen
(Figure 12-32)
2. Dispense manually 300 µl of solution B in cuvette 6 or in any cuvette, indicating it on screen
(Figure 12-33)
3. Dispense manually 300 µl of solution C in cuvette 7 or in any cuvette, indicating it on screen
(Figure 12-34)
4. Dispense manually 300 µl of solution D in cuvette 6 or in any cuvette, indicating it on screen
(Figure 12-35)
5. Wait for 5 minutes to reach thermal stability of the solutions in the cuvette
6. Select
Maintenance
Validations
Photometric Linearity
Cuvette Number
OK
Cuvette Number
OK
Cuvette Number
OK
Cuvette Number
OK
Filter
OK
The instrument makes the reading of four solutions at the selected wavelength,
informing the values in absorbance units AU.
7. Perform the test in duplicate.
8. With the results obtained, make a graph of absorbances found (y) in relation to reference
absorbances (x) and calculate the correlation coefficient by linear regression.
