Abbott CELL-DYN 3000, Руководство оператора

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CELL-DYN

3000 System Operator’s Manual
3-11
9140240E — May 1995
Chapter 3 Principles of Operation
Two populations of cells are clearly seen on the display. The
mononuclear cells fall in the cluster in the lower left corner of the
scatterplot and the polymorphonuclear cells fall in the cluster above and
to the right of them.
The instrument uses a dynamic threshold to determine the best separation
between the two populations. Each cell is then identified as a MONO or a
POLY. Once each cell is identified, it retains this classification no matter
where it appears on other scatterplots.
Figure 3.4: Mononuclear-Polymorphonuclear Scatter
Neutrophil-Eosinophil Separation
The scatter information is plotted with the 90
°
D scatter on the Y axis and
the 90
°
scatter on the X axis. (The 90
°
D/90
°
scatterplot is shown in
Figure 3.5 .) This scatterplot separates the polymorphonuclear cells. The
mononuclear cells have been identified and therefore do not interfere in
the further classification of the polymorphonuclear cells.
Two populations of polymorphonuclear cells are clearly seen on the
display. The neutrophils fall in the lower of the two clusters. The
eosinophils fall in the upper cluster. The instrument uses a dynamic
threshold to determine the best separation between the two populations.
Each cell is then classified as a NEUT or an EOS.
10
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Complexity
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