Abbott CELL-DYN 3000, Руководство оператора

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CELL-DYN

3000 System Operator’s Manual
3-29
9140240E — May 1995
Chapter 3 Principles of Operation
WBC Flags
WBC — displayed next to the WBC result
Cause:
1. The count in a predefined region below the lymphocyte cluster on
the size/complexity (0
°
/10
°
) scatterplot is >10% of the total WBC
count.
2. The count rate monitoring shows a decline in the count rate.
3. The lymphocyte percent is greater than 60% and the position of the
lymphocyte cluster is below a predefined region on the size/
complexity (0
°
/10
°
) scatterplot.
Action: Review a stained smear for the presence of NRBCs. Verify the
WBC count by an alternate method.
NOTE: When the WBC and NRBC flags are set concurrently,
RBCs resistant to the lytic action of the Sheath reagent may be
present. Repeat the sample using the Resistant RBC specimen
type. Specimens with suspected fragile WBC populations should
not be run in the Resistant RBC mode.
DIFF (NLMEB) — displayed next to the BASO result
Cause:
1. A default (preset) value or threshold was used to determine the
five-part differential. This is typically due to the presence of
abnormal cell clusters that the instrument cannot reliably
discriminate between and therefore, a default threshold is selected.
The flag may also be caused by an abnormally low number of cells
in a specific subpopulation.
2. A declining kinetic rate for WBC.
Action: Examine a stained smear to verify the differential values.
NOTE: The DIFF flag is always accompanied by additional
descriptive information, NLMEB, in parentheses. These letters
indicate which subpopulation or group of subpopulations is
suspect when the DIFF flag is displayed. (N=Neutrophils,
L=Lymphocytes, M=Monocytes, E=Eosinophils, B=Basophils.)
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