Abbott CELL-DYN 3000, Руководство оператора

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CELL-DYN

3000 System Operator’s Manual
3-3
9140240E — May 1995
Chapter 3 Principles of Operation
2. The sample segment and sheath are then routed to the WBC mixing
chamber where the dilution is bubble mixed. The final dilution is
1:51.
3. The action of the WBC peristaltic pump transfers the WBC dilution
from the WBC mixing chamber to the sample feed nozzle in the
WBC flow cell.
4. The WBC metering syringe injects 78 µL of the WBC dilution into
the flow cell sheath stream.
5. A laser beam is focused on the flow cell. As the sample stream
intersects the laser beam, the light scattered by the cells is
measured at four different angles.
RBC/PLT Analysis
1. The RBC Diluent syringe dispenses 8.0 mL of Diluent through the
shear valve, where it picks up the 0.64 µL RBC/PLT segment.
2. The sample segment and diluent are then routed to the mixing
chamber of the RBC/PLT transducer assembly, where the dilution
is bubble mixed. The final dilution is 1:12,501.
3. The dilution is pulled through the aperture by vacuum. The
volumetric metering process ensures that 100 µL of the dilution are
used for the measurement. Electrical impedance is used to count
the RBCs and PLTs as they traverse the aperture.
Hemoglobin Analysis
1. The 12 µL HGB sample segment is transported by 1.5 mL of
Diluent and 1.5 mL Hemoglobin Lyse reagent to the HGB mixing
chamber. The 1:251 dilution is bubble mixed, then transferred to
the HGB flow cell.
2. In the flow cell, the absorbance of the sample is read at 540 nm.
The absorbance is directly proportional to the HGB concentration
of the sample.
The flow cell is drained and filled with Hemoglobin Lyse reagent
for a blank (reference) reading.
Results Displayed
All data is transmitted to the Data Station for analysis. Results are
computed for all parameters and are displayed on the
Data Station RUN
Screen . Results are also stored in a log format called the data log .
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