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3-12
CELL-DYN
3000 System Operator’s Manual
9140240E — May 1995
Principles of Operation Chapter 3
All cells scatter a certain amount of 90
°
D light. The eosinophils scatter
more 90
°
D light than any of the other cells because of the unique nature
of granules they contain. This property of the eosinophils is used to
positively identify them and thus clearly differentiate them from the
neutrophil population.
Figure 3.5: Neutrophil-Eosinophil Scatter
Mononuclear Separation
The scatter information is plotted with the 0
°
scatter on the Y axis and
the 10
°
.) The mononuclear cells are plotted on this scatterplot. The
algorithm also uses the orientation of the neutrophil cluster to aid in
classifying the mononuclears. Three populations of mononuclear cells
are clearly seen on the display.
There are three populations of mononuclears because basophils are
included in the mononuclear cluster. Typically, basophils are granulated
cells and therefore more complex than the mononuclear cells. However,
the basophilic granules are water soluble and dissolve in the Sheath
reagent. Consequently, the degranulated basophil becomes a less
complex cell that falls into the mononuclear cluster.
90
°
Orthogonal
9
0
°
D
e
p
o
l
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Содержание CELL-DYN 3000
Страница 31: ...10 CELL DYN 3000 System Operator s Manual 9140240E May 1995 NOTES Search Go Back Book TOC ...
Страница 265: ...6 10 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Calibration Chapter 6 NOTES Search Go Back Book TOC ...
Страница 287: ...6 32 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Calibration Chapter 6 NOTES Search Go Back Book TOC ...
Страница 313: ...6 58 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Calibration Chapter 6 NOTES Search Go Back Book TOC ...
Страница 347: ...6 92 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Calibration Chapter 6 NOTES Search Go Back Book TOC ...
Страница 351: ...7 2 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Quality Control Chapter 7 NOTES Search Go Back Book TOC ...
Страница 369: ...7 20 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Quality Control Chapter 7 NOTES Search Go Back Book TOC ...
Страница 371: ...7 22 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Quality Control Chapter 7 NOTES Search Go Back Book TOC ...
Страница 387: ...9 6 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Maintenance Chapter 9 NOTES Search Go Back Book TOC ...
Страница 405: ...9 24 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Maintenance Chapter 9 NOTES Search Go Back Book TOC ...
Страница 431: ...10 2 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Troubleshooting Chapter 10 NOTES Search Go Back Book TOC ...
Страница 473: ...10 44 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Troubleshooting Chapter 10 NOTES Search Go Back Book TOC ...
Страница 495: ...10 66 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Troubleshooting Chapter 10 NOTES Search Go Back Book TOC ...
Страница 509: ...10 80 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Troubleshooting Chapter 10 NOTES Search Go Back Book TOC ...
Страница 535: ...12 16 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Sample Loader Chapter 12 NOTES Search Go Back Book TOC ...
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