CELL-DYN
®
3200 System Operator’s Manual
3-1
9140181H—October 2001
Section 3
Principles of Operation
Section 3
Principles of Operation
Overview
The principles the CELL-DYN 3200 uses to measure, count and calculate the
hematological parameters are discussed in Sample Analysis Cycle Overview and
Introduction to Flow Cytometry within this section. Subsequent sections discuss
the measurement process for WBC, RBC, PLT, and HGB. The last subsection,
Operational Messages and Data Flagging, discusses the flags generated by the
instrument due to measured parameters outside predefined limits, sample
abnormality, interference in the measurement process, or detection of an abnormal
subpopulation. Quality Control methodology is discussed in
. Reticulocytes and Reticulocyte flagging are discussed in
The two independent measurement channels used in the CELL-DYN 3200 are:
• The Optical channel for determining the WBC, NOC, and RBC/PLT data
• The Hemoglobin channel for determining the HGB
During each instrument cycle, the sample is aspirated, diluted, and mixed before
each parameter is measured.
NOTE:
An increase in flags will occur in RRBC and FWBC modes.
Sample Aspiration
There are two modes of whole blood sample aspiration on the CELL-DYN 3200.
The operator selects the mode of aspiration from the
RUN
screen.
• The
Open Sampler Mod
e is used to aspirate the sample from a collection
tube that has been opened and is held under the open sample aspiration probe.
• The
Closed Sampler Mode
is used to aspirate the blood directly from a closed
collection tube by piercing the tube stopper. On the CS model, sample tubes are
processed manually. On the SL model, sample tubes are processed
automatically using the Sample Loader.
The aspiration volumes are:
Open Mode
150 µL ± 10%
Closed Mode (CS)
240 µL ± 10%
Sample Loader (SL) 240 µL ± 10%
Once the mode of aspiration is selected, the whole blood sample is aspirated to the
Shear Valve by vacuum/pressure action. An ultrasonic sensor, located upstream of
the Shear Valve, checks the integrity of the sample stream before it enters the Shear
Valve. An LED sensor, located downstream of the Shear Valve, checks the sample
stream to ensure the proper amount of blood has been transferred through the Shear
Valve.
Содержание CELL-DYN 3200
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Страница 52: ...1 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Use or Function Overview Section 1 NOTES ...
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Страница 154: ...5 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Overview Section 5 NOTES ...
Страница 234: ...5 82 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Run Menu Section 5 NOTES ...
Страница 300: ...6 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures Overview Section 6 NOTES ...
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Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
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Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
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Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
Страница 662: ...A 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Overview Appendix A NOTES ...
Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...