Abbott CELL-DYN 3200, Руководство оператора системы

Страница: 657 / 684

CELL-DYN ® 3200 System Operator’s Manual
14-69
9140181K—July 2002
Section 14 Reticulocyte Package
Data Invalidating Alerts
The Reticulocyte results are not suppressed for the Data Invalidating Alerts. The
alert message appears in the lower left-hand quadrant of the Display Monitor
screen and on the graphics printout under the heading
ALERTS
.
High Background Counts
NOTE: The background count should be less than 100 counts.
1. If the background count is high, repeat the run.
2. If the results are still unacceptable, open a new tube of reticulocyte reagent
and repeat the background count.
3. If the background count is still unacceptable, run a tube from a new lot of
reticulocyte reagent if available.
4. If the results are still unacceptable, while in the Reticulocyte Background
mode, press the Touch Plate to cycle air through the system.
5. If the results are still unacceptable, exit the Reticulocyte Package and
perform a background check in the Standard Hematology mode.
6. If the background in the Standard Hematology mode is unacceptable, see
Section 10: Troubleshooting and Diagnostics ; Subsection:
Troubleshooting High Background Counts .
Table 14.3 Data Invalidating Alerts
Alert Probable Cause Corrective Action
Fragile RBCs
NOTE: Alert occurs when the
average count rate rapidly
decreases during the
Reticulocyte count cycle.
· Air bubble
· Staining a fragile RBC
specimen too long in the
reticulocyte reagent
· Fragile RBCs
1. Run a background count to cycle air
through the system. Rerun the
Reticulocyte specimen.
2. Prepare another dilution verifying proper
specimen preparation as discussed in
Routine Operation, Reticulocyte
Specimens, Running Specimens,
Patient, Specimen Preparation
within
this chapter. Run the dilution after
adequate incubation as indicated in the
Reagent package insert.
3. Verify Reticulocyte results by an alternate
method.
Excessive RBC Loss (ERL) · Specimen staining time
too long in the
reticulocyte reagent
·
Rapid degeneration of
RBCs.
·
High concentration of
platelets, platelet
aggregates, or other
interfering substances.
· Microcytic RBCs
· Improper instrument
settings
1. Prepare another dilution verifying proper
specimen preparation as discussed in
Routine Operation, Reticulocyte
Specimens, Running Specimens,
Patient, Specimen Preparation
within
this chapter, and run after adequate
incubation as indicated in the Reagent
package insert.
2. Rerun the specimen.
3. If the flag persists, verify the reticulocyte
results by an alternative method.