Abbott CELL-DYN 3200, Руководство оператора системы

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CELL-DYN ® 3200 System Operator’s Manual
9140181H—October 2001
Performance Characteristics and Specifications
Performance Specifications
Section 4
Reticulocyte % measurement carryover, as shown in Table 4.7, is calculated using
actual Listmode counts, rather than using the Reticulocyte percent. It is calculated
using the following formula:
Reticulocyte carryover is determined on fresh whole blood samples with RBC in
the range 4.0–6.0 M/µL. Retic Background Count is reported on the Retic Run
Results Screen, while Retic Listmode can be found in DIAGNOSTICS
→
RETIC
RAW DATA.
Precision
Precision is the measurement of how closely the instrument is able to reproduce the
same results from repeated runs of the same sample.
Table 4.8 and Table 4.9 present the limits of acceptable precision for specimens run
in both the Open and Closed modes. The stated CV% in these tables represents the
instrument’s precision at a 95% confidence level from N=20 replicate runs based
on the same sample.
NOTE: 95% confidence limit means that at least 19 of the 20 determinations meet
this limit.
NOTE: N=20 is based on one whole blood sample aliquotted into 4 tubes, with
each tube run 5 times in Closed mode.
Fresh whole blood samples that are used to verify precision specifications should
have results that fall within the laboratory’s normal range. These samples should
not display any of the following suspect parameter flags:
WBC
LRI
URI
LURI
The precision values listed in this section are applicable to Patient, Fragile WBC,
Resistant RBC (Open mode only), and Reticulocyte RBC (Open Mode Only)
specimen types.
Table 4.7 Carryover for Reticulocyte
Parameter Specimen Range Carryover
RETIC % 1.8%–20%
with 30,000 Listmode events < 0.5%
% Carryover =
Background
1
-
Background
3
Retic Listmode
3
- Background Count
3
X 100