Abbott CELL-DYN 3200, Руководство оператора системы

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CELL-DYN ® 3200 System Operator’s Manual
14-59
9140181H—October 2001
Section 14 Reticulocyte Package
Reticulocyte Specimens
Overview
This subsection discusses routine operation of the Reticulocyte Package.
Guidelines and procedures are provided for running background counts, quality
control, and patient specimens. Proper start-up procedures should be performed
prior to processing patient specimens. The Reticulocyte Package is only available
for use in the Open Sampler Mode.
For background counts, a tube of reticulocyte reagent is run without an aliquot of
whole blood, to check for particulate material in the reagent and system.
Control material should be properly warmed and mixed according to the
manufacturer’s recommendations. Patient reticulocyte controls should be run and
handled according to the laboratory’s protocol. Quality control checks (which
verify Reticulocyte Package performance) should be performed on each shift that
reticulocyte samples are run according to the laboratory’s protocol.
Each reticulocyte sample is run by starting from the
RETIC SPECIMEN TYPE
screen.
The operator selects the type of Reticulocyte specimen to be run (Patient, QC, or
Background) and proceeds through the screen(s) displayed for that specimen type.
When the reticulocyte sample is completed, the
[NEXT RETIC]
key is displayed.
When the
[NEXT RETIC]
key is pressed, the operator can then select the specimen
type for the next specimen. Specific instructions for each specimen type are given
later in this section.
CAUTION: When using the reticulocyte reagent, avoid contact with skin
and clothing. This reagent contains New Methylene Blue, which will stain
skin, clothing, and many other surfaces.
WARNING: Potential Biohazard. Consider all clinical specimens and
controls that contain human blood or serum as potentially infectious. Use
established, good laboratory working practices when handling these
specimens. Wear appropriate personal protective equipment and follow
other biosafety practices as specified in the OSHA Bloodborne Pathogen
Rule or other equivalent biosafety procedures.
Specimen Requirements
A fresh non-hemolyzed, whole blood specimen collection in K
2
EDTA, K
3
EDTA
or NA
2
EDTA is the specimen of choice for Reticulocyte analysis on the
CELL-DYN 3200 System.
Specimens may be run up to 8 hours after collection time when stored at room
temperature.
NOTE: Studies have shown that reticulocytes continue to mature at room
temperature. Increased flagging can occur when using specimens more
than 8 hours old.
If a delay in analysis is anticipated, specimens may be processed up to 72 hours if
stored at refrigerated temperature.