Abbott CELL-DYN 3200, Руководство оператора системы

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CELL-DYN ® 3200 System Operator’s Manual
9140181H—October 2001
Use or Function
System Components
Section 1
CELL-DYN Reagents
CELL-DYN 3200 Diluent/Sheath (List Number 03H79-01)
CELL-DYN Diluent/Sheath is formulated to meet the following requirements:
• Act as the diluent for the RBCs, PLTs, and hemoglobin
• Maintain the stable diluted cell volume of each red cell and platelet during
the count and sizing portion of the measurement cycle
• Serve as a sheath fluid for the hydrodynamic focusing process
• Serve as a rinsing agent for the fluidics system
• Provide background counts equal to or less than:
WOC: 0.10 x K/µL
NOC: 0.10 x K/µL
RBC: 0.02 x M/µL
HGB: 0.10 x g/dL
PLT: 5.0 x K/µL
CELL-DYN 3200 CN-Free HGB/NOC Lyse (List Number 03H80-02)
CELL-DYN CN-Free HGB/NOC Lyse is formulated to meet the following
requirements:
• Rapidly lyse the red blood cells and minimize the resultant stroma
• Strip the white cell cytoplasm leaving the nuclear membrane intact so the
white cell nuclei can be enumerated
• Convert hemoglobin to a stable chromagen complex that is measurable at
555 nm.
CELL-DYN 3200 WBC Lyse (List Number 03H78-01)
CELL-DYN WBC Lyse is formulated to meet the following requirements:
• Act as the diluent for the WBCs
• Osmotically lyse the red cells
• Maintain the light scattering properties of the WBCs for the duration of the
measurement period
• Provide sufficient wetting action to prevent accumulation of air bubbles in
the WBC flow system
• Maintain a WOC background count equal to or less than 0.10 x K/µL
• Act as a diluent for Reticulocytes