6-44
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Enter Factor Method
Section 6
6. Calibrating the SL model with Calibrator or Fresh Whole Blood:
a. Determine the number of runs you expect to make (6 runs should be the
minimum for calibrator, 10 runs for fresh whole blood) and obtain the
same number of empty 5-mL tubes containing no anticoagulant.
b. Mix the specimen well. If Calibrator, follow the directions on the
package insert. If Fresh Whole Blood, invert the sample ten times. Do not
shake the specimen.
c. Aliquot the sample as follows:
1. For Calibrator, aliquot the sample in equal volumes into the tubes
(each tube should contain a minimum of 1.5 mL of sample).
2. For Fresh Whole Blood, there should be a minimum of 5 samples
(refer to
Number of Cycles
under
Calibration Materials
earlier in
this section). Aliquot the first sample in equal volumes into two
tubes. Aliquot the second sample in equal volumes into another two
tubes. Repeat this procedure until all 5 samples have been aliquoted
(for a total of 10 tubes).
d. Place the closed tubes on a rack and set the rack on the “load” side.
e. Press
[START LOADER]
. Allow the instrument to process all the samples.
7. When sample processing is completed, press
[MAIN]
followed by
[QUALITY
CONTROL]
. Use the arrow keys to place the cursor on the appropriate QC file
and press
[VIEW QC LOG]
.
8. Press
[PRINT QC LOG]
to print the Summary Report for the selected QC file.
9. To determine the Mode to Mode Calibration Difference for each parameter:
a. Transfer the information from Step 8 above to the Manual Mode to Mode
Calibration Worksheet and enter it in the first column. (Worksheet 4)
b. Determine the Open Mode Mean for the Calibrator or Fresh Whole
Blood samples as described in the
Calibration Procedure - Open Mode,
Determining New Calibration Factors,
steps 2–8. Transfer the
information to the Manual Mode to Mode Calibration worksheet and
enter it in columns 2 and 3.
c. Calculate the Mode to Mode Calibration Difference for each parameter
as follows:
NOTE:
Make copies of this worksheet as needed.
10. Enter the Mode to Mode Calibrator Difference for each parameter in column
4 of the worksheet.
Closed Mode Mean - Open Mode Mean
Open Mode Mean
x
100
= % Difference
Содержание CELL-DYN 3200
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Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
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Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
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Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
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Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...