Beckman Coulter Multisizer 4, User Manual

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PN A51387A
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Installing and Calibrating an Aperture Tube
Using Small Aperture Tubes (20 µm and 30 µm)
Using Small Aperture Tubes (20 µm and 30 µm)
Small apertures (20 µm and 30 µm) are fragile and require special handling, cleaning, and operating
procedures.
Because small apertures measure extremely small particles, it is necessary to take steps to safeguard
the aperture tube and reduce electrical noise.
• Handle the aperture tube properly
•
Remove environmental factors that can contribute to electrical noise
• Pre-filter cleaning fluids and electrolyte
•
Clean the system thoroughly prior to using a small aperture tube
• Clean ST Beakers, Accuvette® STs, jars, ST Aperture Tubes, and other accessories
•
Run a noise profile to verify a low background count
• Use appropriate current and gain settings
•
Store the aperture tube properly
• Adhere to nano-particle calibration standards, if applicable
Handling Small Aperture Tubes
Always wear powder-free latex or nitrile gloves when handling small aperture tubes and other
system components and accessories, such as the Electrolyte Jar, ST Beakers, and Accuvette® STs.
Hold the aperture tube near the top during installation and removal ( Figure 3.1 ). Avoid touching the
aperture tube at the bottom, near the orifice.
Figure 3.1 Proper Handling of a Small Aperture Tube