Beckman Coulter Multisizer 4, User Manual

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PN A51387A
B
4-7
Selecting Analysis Settings: SOM and SOP
Using a Standard Operating Method (SOM)
4
Table 4.3 Control Mode (Step 2): Selections
Select and specify... To end the run when...
Time The specified number of seconds have elapsed since the run began. Enter
any time from 1 to 999 seconds, or select a number from the drop-down list.
When you click
(
Next
)
, the valid range is displayed.
Time mode uses constant pressure to move sample through the orifice for
a pre-determined length of time.
Volumetric The specified volume of electrolyte has passed through the aperture tube.
Enter a volume from 50 to 2000 µL, or select a number from the drop-down
list. When you click
(
Next
)
, the valid range is displayed.
Use Volumetric mode to move a precise volume of sample through the
orifice of the aperture tube with a metering pump.
NOTE
Volumetric mode is not allowed on aperture sizes greater than 360 µm. The
largest standard aperture size allowed is 280 µm.
Total Count The specified number of particles or cells (corrected for coincidence) has
passed through the aperture. Enter a number from 50 to 500,000.
NOTE
To specify particles or cells, exit the SOM Wizard and select
SOP
>
Edit
Preferences
on the Main Menu bar. In the Edit Preferences window, select
the View Options tab and under Measuring, select Particles or Cells. To save
changes you made in the Wizard, go to step 9 and click
(
Save
)
before
exiting. Re-open the Wizard and return to Control Mode, step 2.
Modal Count The specified number of particles or cells has accumulated in the modal
channel. Enter a number from 10 to 100,000.