Beckman Coulter Multisizer 4, User Manual

Page: 114 / 238

PN A51387A
B
4-24
Selecting Analysis Settings: SOM and SOP
Using a Standard Operating Method (SOM)
• Stirrer. Use the Stirrer tab to enter stirrer settings or disable stirrer buttons on the Analyzer
Control Panel for the duration of a run.
For more information on the Stirrer window as it appears in the SOM Wizard, see Standard
Operating Method: Stirrer Settings
, page 4-10 .
CAUTION
The Multisizer 4 will automatically position the stirrer based on the Smart
Technology Beaker size you select in the SOM.
• Current & Gain. Enter the lower limit of a size distribution as well as the electrical pulse size
(current) and amplification (gain) that the Analyzer will use as particles pass through the
aperture.
For more information on the Current and Gain window as it appears in the SOM Wizard, see
Standard Operating Method: Threshold, Current and Gain , page 4-13 . For information on additional
features available in the Edit the SOM window, see Using the Current & Gain Tab in the Edit the
SOM Window , page 4-14 .
• Pulse to Size Settings. Enter the number of bins (channels), bin spacing, and other information
that will determine the range and resolution of analysis results. After running the analysis, you
can change these settings by selecting
Calculate > Convert Pulses to Size
on the Main Menu bar.
For more information on the Pulse to Size Settings window as it appears in the SOM Wizard, see
Standard Operating Method: Pulse to Size Settings , page 4-15 .
• Concentration.
Enter information about sample volume, density, and dilution.
For more information on the Concentration window as it appears in the SOM Wizard, see
Standard Operating Method: Concentration Information , page 4-17 .
• Blockage. Enter settings that will alert the software to an aperture blockage and select steps the
Multisizer 4 will follow when it detects a blockage.
For more information on the Blockage window as it appears in the SOM Wizard, see Standard
Operating Method: Blockage Detection , page 4-18 .
CAUTION
Do not attempt to unblock the aperture using an object or your finger. The
Multisizer 4 unblock procedure uses a reverse flow of electrolyte through the
aperture orifice to remove stuck particles. Any other method of removing the
trapped particles could damage the aperture. For more information, see
Handling
Small Aperture Tubes , page 3-4 .
Click
(
Save SOM
)
, available on every tab, to save the current SOM settings.