Beckman Coulter Multisizer 4, User Manual

Page: 56 / 238

PN A51387A
B
3-2
Installing and Calibrating an Aperture Tube
Installing an Aperture Tube
Installing an Aperture Tube
This section describes how to correctly place an aperture tube in the Analyzer. After installing the
aperture tube, you will need to:
• Calibrate the aperture
•
Follow the step-by-step guide provided in the Change Aperture Tube Wizard. The Change
Aperture Tube Wizard allows you to verify calibration, test aperture settings, and enter the new
aperture tube size and serial number in the Multisizer 4 software.
To install an aperture tube:
1.
Clean the new ST Aperture Tube. For instructions on the proper aperture cleaning procedure,
see
Cleaning the Aperture Tube, Beakers, and Accuvette® STs , page 1-15 .
Table 3.1 Aperture Sizes
Aperture
(Nominal
Diameter,
µm)
Diameter Range (µm)
Volume Range µm
3
or fL
Total Standard Extended Total Standard Extended
20* 0.4–16 0.4–12 12–16
0.034–2.14
×
10
3 0.034–905
905–2.14
×
10
3
30* 0.6–24 0.6–18 18–24
0.113–7.24
×
10
3
0.113–3.05
×
10
3
3.05
×
10
3
–7.24
×
10
3
50 1.0–40 1.0–30 30–40
0.524–33.5
×
10
3
0.524–14.1
×
10
3
14.1
×
10
3
–33.5
×
10
3
70 1.4–56 1.4–42 42–56
1.44–92.0
×
10
3
1.44–38.8
×
10
3
38.8
×
10
3
–92.0
×
10
3
100 2.0–80 2.0–60 60–80
4.19–268
×
10
3
4.19–113
×
10
3
113
×
10
3
–268
×
10
3
140 2.8–112 2.8–84 84–112
11.5–736
×
10
3
11.5–310
×
10
3
310
×
10
3
–736
×
10
3
200 4.0–160 4.0–120 120–160
33.5–2.14
×
10
6
33.5–905
×
10
3
905
×
10
3
–2.14
×
10
6
280 5.6–224 5.6–168 168–224
92.0–5.88
×
10
6
92.0–2.48
×
10
6
2.48
×
10
3
–5.88
×
10
6
400 8.0–320 8.0–240 240–320
268–17.2
×
10
6
268–7.24
×
10
6
7.24
×
10
6
–17.2
×
10
6
560 11.2–448 11.2–336 336–448
736–47.1
×
10
6
736–19.9
×
10
6
19.9
×
10
6
–47.1
×
10
6
1000** 20–800 20–600 600–800
4189–268
×
10
6
4189–113
×
10
6
113.1
×
10
6
–268
×
10
6
2000** 200–1600 200–1200 1200–1600
4.19
×
10
6
–2.14
×
10
9
4.19
×
10
6
–905
×
10
6
905
×
10
6
–2.14
×
10
9
* Range depends on system cleanliness and environmental electrical noise.
** Range depends on sample density.