Beckman Coulter Multisizer 4, User Manual

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PN A51387A
B
1-17
Analyzer Overview
Pre-Filtering the Electrolyte and Cleaning Fluid
1
Clean the system with 98% Isopropyl Alcohol:
9. Fill the Electrolyte Jar with 98% Isopropyl Alcohol.
10. Repeat Steps 5 through 8 above.
Clean the system with de-ionized water:
11. Rinse and fill the Electrolyte Jar with de-ionized water.
12. Repeat Steps 5 through 8 above.
Flush the system:
13. Fill the Electrolyte Jar with filtered ISOTON® II.
14. On the Multisizer 4 software Instrument Toolbar, click the
(
Flush
)
button to flush the aperture
tube.
15. Flush the aperture tube three times.
Pre-Filtering the Electrolyte and Cleaning Fluid
When working with small apertures, it is necessary to pre-filter de-ionized water and/or ISOTON® II
used to fill, flush, or clean the system and accessories.
Filter the Isoton II or diluent solution with a compatible 0.2-µm filter. A 0.1-µm filter can also be
used in series with a 0.2-µm filter for additional filtering. Contact a filtration supplier to determine
the appropriate filtration components for your application. One possible configuration for filtering
large amounts of diluent is shown in the diagram below (
Figure 1.10 ).
Figure 1.10 Closed Circuit Filtration System