Beckman Coulter Multisizer 4, User Manual

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PN A51387A
B
3-3
Installing and Calibrating an Aperture Tube
Installing an Aperture Tube
3
CAUTION
Small apertures (20 µm and 30 µm) are fragile. Beckman Coulter recommends
that you handle them with great care. For more information on small aperture
handling, cleaning, and operating procedures, see
Using Small Aperture Tubes
(20 µm and 30 µm) , page 3-4 .
2.
If you have not already done so, power on the Analyzer, open the Multisizer 4 software, and
connect the software to the Analyzer.
3.
In the Multisizer 4 software, select
Run > Change Aperture Tube Wizard
on the Main Menu bar.
4. In the Change Aperture Tube Wizard, click
(
Aperture Tube
)
. The Select Aperture Tube window
opens. For more information, see Step 1: Select the new aperture tube , page 3-23 .
5. Hold the ST Aperture Tube bar code in front of the Bar Code Reader on the Control Panel.
• If an operator has already calibrated the aperture and entered ST Aperture Tube
information in the software, the software highlights the aperture tube size, serial number,
and Kd. Click
(
OK
)
.
• If the ST Aperture Tube has not been entered into the system, the software displays the
aperture tube size and serial number. To enter this information, use the Add New Aperture
Tube window.
6. Open the Sample Compartment door.
7. Lower the platform to the loading position using the platform release (see Platform Release ,
page 1-11 ).
8. Turn the aperture tube knob in either direction until it points down. The aperture knob is
locked only when the knob points up (12 o’clock position).
9. If necessary, remove the currently installed ST Aperture Tube, and click
(
Next
)
.
10.
Insert the new ST Aperture Tube in the connection head with the logo facing you. Insert the
tube as far as possible (0.88 in/22 mm).
CAUTION
Do not grease the aperture tube. The Multisizer 4 aperture tube connection forms
a greaseless seal with O-rings.
11. Turn the aperture tube knob until it points up in the vertical position. Turning the knob until
it is precisely vertical (in the 12 o’clock position) locks the aperture tube in place.
12. Return to the Change Aperture Tube Wizard and proceed through the remaining steps.