Beckman Coulter Multisizer 4, User Manual

Page: 82 / 238

PN A51387A
B
3-28
Installing and Calibrating an Aperture Tube
The Change Aperture Tube Wizard
Step 6: Fill the System
When you install a new aperture tube, it is necessary to remove air from the system. The system fills
from the Electrolyte Jar into the Waste Tank. (Electrolyte does not pass through the aperture orifice
unless you are using the Unblock function to clear the orifice.) This procedure is similar to flushing
the system to remove particles.
To fill the system:
1. In the Change Aperture Tube Wizard, click
(
Next
)
until you reach the step Fill the system if it
is not already highlighted.
2.
In the Status Panel, verify that the Waste Tank is empty. If the Waste Tank is not empty, exit the
Change Aperture Tube Wizard and click
(
Empty
)
in the Instrument Toolbar. Re-open the
Wizard and click
(
Next
)
until the
(
Fill System
)
button is selected.
3. To the right of the Fill the system step, click
(
Fill System
)
.
4. The Status Panel displays progress while the system is filling.
5.
When the process is complete, the Status Panel displays READY and the Wizard automatically
highlights the next step, Adjust the metering pump (page
3-28 ).
Step 7: Adjust the Metering Pump
This step appears in the Change Aperture Tube Wizard only if you are using an aperture tube
smaller than 400 µm. The metering pump is required only when using volumetric mode. Aperture
tubes 400 µm and larger can be used only in time and count modes.
For information on control modes (volumetric, time, and count) see Standard Operating Method:
Control Mode , page 4-6 .
To adjust the metering pump:
1. In the Change Aperture Tube Wizard, click
(
Next
)
until you reach the Adjust the metering
pump step if it is not already highlighted.
2. To the right of the Adjust the metering pump step, click
(
Metering Pump
)
.
3. The Status Panel displays Adjusting Metering Pump.
4. When the process is complete, the Status Panel displays READY and the Wizard automatically
highlights the next step, Set current and gain (page
3-28 ).
Step 8: Set Current and Gain
Setting current and gain determines the electrical pulse size (current) and amplification (gain) that
the Analyzer will use as particles pass through the aperture. Unless you are running Service Mode
(page
9-18 ), the Multisizer 4 software sets current and gain automatically.