Beckman Coulter Multisizer 4, User Manual

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PN A51387A
B
7-11
Working with Analysis Files
Viewing Analysis Files
7
d. In the Options pane, select one or more of the following, if desired:
• Pulse Edit . Select this option if you have a narrow distribution and want to ensure
accurate analysis results by eliminating large pulses. Particles that do not pass through
the center of the aperture tend to produce wider pulses. Pulse edit removes the widest
pulses from statistical analysis and displayed results.
• Use Shape Factor . Select this option if you want to match your results to another
particle sizing instrument, or if you need to adjust analysis results based on a known
factor. Entering a shape factor of 1.03, for example, increases particle diameter or
volume by 3%.
e. Click
(
OK
)
.
Converting Pulses to Size Between Cursors
To convert pulses to particle size between two cursor points on a graph:
1. Select bin size and other options in the Convert Pulses to Size Settings window (page 7-9 ).
2.
In the graph view, place two cursors to delimit analysis results. For information on inserting
cursors, see
Using Cursors in Graphs , page 7-3 .
3. Select
Calculate > Convert Pulses between Cursors
on the Run Menu bar. The graph view
changes to display only results between the two cursor points.
NOTE
Convert Pulses between Cursors
allows you to select a range of particle sizes for analysis based
on cursor points. You can also enter specific diameter or volume limits in the Convert Pulses to
Size Settings window. Limiting the particle size range using either cursors or text input allows
you to increase the precision of your analysis by viewing the same number of size bins within a
smaller distribution.
Converting Pulses to Size for the Widest Distributions
To automatically analyze the widest possible distribution based on aperture size, select
Calculate >
Convert Pulses (2% to 60%)
, or, if you’re using
a
2000-µm aperture tube, select
Calculate > Convert
Pulses (10% to 60%)
.
To change the number of bins or other options associated with converting pulses, select
Calculate >
Convert Pulses to Size
on the Run Menu bar. Enter new settings in the Convert Pulses to Size Settings
window (page 7-9 ).