Beckman Coulter Multisizer 4, User Manual

Page: 5 / 238

v
Table of Contents
Main Toolbar Buttons: Fourth Group , 2-8
Status Panel , 2-8
Viewing Area , 2-10
Run Menu , 2-10
Instrument Toolbar Buttons , 2-11
Instrument Toolbar: Run Buttons , 2-11
Instrument Toolbar: Reset Button , 2-12
Instrument Toolbar: Fluid Control Buttons , 2-12
Status Bar , 2-12
CHAPTER 3:
Installing and Calibrating an Aperture Tube , 3-1
Overview , 3-1
When to Install a New Aperture Tube , 3-1
Selecting an Aperture Size , 3-1
Installing an Aperture Tube , 3-2
Using Small Aperture Tubes (20 µm and 30 µm) , 3-4
Handling Small Aperture Tubes , 3-4
Unblocking small aperture tubes , 3-5
Reducing Environmental Factors That Create Noise , 3-5
Create a dust-free lab environment , 3-5
Reduce acoustic noise levels , 3-5
Reduce vibration , 3-5
Block electrical interference , 3-5
Pre-Filtering the Electrolyte and Cleaning Fluid , 3-5
Cleaning the Analyzer Prior to Using a Small Aperture , 3-6
Cleaning System Components and Accessories , 3-6
Small Aperture Noise Profiles , 3-7
Using Appropriate Current and Gain Settings , 3-9
Storing Small Aperture Tubes , 3-9
Calibrating the Aperture , 3-10
Choosing the Calibrator Size , 3-10
Nano-Size Particle Calibration Standards , 3-11
Selecting Calibration Options , 3-11
Preparing the Analyzer for Calibration , 3-12
Running the Calibration , 3-13
Pausing or Stopping Calibration , 3-14
Completing the Calibration Run (Accepting the Kd) , 3-15
Obtaining the Mean Kd , 3-16
Example , 3-17
Updating the Mean Kd Value , 3-17
Verifying the Calibration , 3-18
Saving the Calibration File , 3-21
The Change Aperture Tube Wizard , 3-21
Step 1: Select the new aperture tube , 3-23