Beckman Coulter Multisizer 4, User Manual, Page: 60 / 238

PN A51387A
B
3-6
Installing and Calibrating an Aperture Tube
Using Small Aperture Tubes (20 µm and 30 µm)
Figure 3.2 Closed-Circuit Filtration System
Cleaning the Analyzer Prior to Using a Small Aperture
Before running analyses with a small aperture tube, clean the system. An effective cleaning
procedure includes filling and draining the system multiple times and in multiple cycles,
alternating between pre-filtered, de-ionized water and 98% isopropyl alcohol. For step-by-step
instructions, see
Cleaning the Analyzer
, page 1-16 .
Cleaning System Components and Accessories
Before operating the system, rinse all system components and accessories in pre-filtered ISOTON® II
(see
Pre-Filtering the Electrolyte and Cleaning Fluid
, page 3-5 ).
System components and accessories include:
•
Electrolyte Jar
• Aperture Tube
• Electrode
• Particle Trap
• ST Beaker or Accuvette® ST
When handling small aperture tubes and other system components, wear powder-free latex or
nitrile gloves.
To clean the aperture tube and electrode:
1. Press the platform release towards the back of the Sample Compartment and lower the platform
to the loading position.
2. Use a squeeze bottle filled with clean or pre-filtered ISOTON® II to rinse the aperture tube,
external electrode, and the stirrer (
Figure 3.3 , below).
3. Select a beaker that is at least one size larger than the size you used for the previous run (or a
400 mL beaker if you used that size for the previous run).