PROCEDURE MANUAL FOR THE i-STAT SYSTEM
5
REV. DATE: 16-Oct-12
ART: 714446-00L
Test Sample collected without anticoagulant immediately. Test samples for ACT, lactate and PT/INR immediately. Test
samples for pH,
P
CO2, TCO
2
and ionized calcium within 10 minutes of sample draw. If not tested immediately, remix the
sample before testing and discard the first two drops of blood from a syringe before testing. For the glucose test strip and
other cartridge tests, test sample within 30 minutes of collection.
Finger and Heelstick Specimens
For tests other than PT/INR, wipe away the first drop of blood, which contains excess tissue fluid which can increase the
potassium result and decrease other test results. Avoid drawing air into the capillary tube. Use balanced heparin or plain
capillary tubes for ionized calcium. Test samples immediately to avoid clotting (especially in neonates).
Capillary samples
are NOT recommended for ACT, Troponin I/cTnI, CK-MB and BNP.
Criteria For Specimen Rejection
Evidence of clotting
Specimens collected in vacuum tubes with anticoagulant other than lithium or sodium heparin (or EDTA
for BNP or glucose cartridges).
Specimens for ACT or PT/INR collected in glass syringes or tubes or with anticoagulant of any kind
Syringe for pH,
P
CO
2
,
P
O
2
and TCO
2
with air bubbles in sample
Incompletely filled vacuum tube for the measurement of ionized calcium,
P
CO
2
, HCO
3
or TCO
2
Other sample types such as urine, CSF, and pleural fluid
Precautions: Avoid the Following Circumstances
Drawing a specimen from an arm with an I.V.
Stasis (tourniquet left on longer than one minute before venipuncture)
Extra muscle activity (fist pumping)
Hemolysis (alcohol left over puncture site, or a traumatic draw)
Icing before filling cartridge
Time delays before filling cartridge, especially lactate, ACT, and PT/INR
Exposing the sample to air when measuring pH,
P
CO
2
,
P
O
2
and TCO
2
.
PROCEDURE FOR ANALYSIS
Preparation for Use
An individual cartridge may be used after standing 5 minutes, in its pouch, at room temperature. An entire box should stand
at room temperature for one hour before cartridges are used.
Summary of Contents for i-STAT 1
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