Art: 714372-00L
Rev. Date: 30-Jul-12 10-3
i-STAT cTnI and CK-MB cartridges require the use of either:
1. heparinized whole blood or plasma samples collected in syringes or
evacuated tubes containing lithium or sodium heparin, or
2. non-heparinized whole blood samples tested within one minute of
drawing from a patient into a plastic syringe or plastic evacuated tube
containing no additives.
The use of whole blood or plasma samples containing other
anticoagulants such as EDTA, oxalate, and citrate will cause
deactivation of the alkaline phosphatase, resulting in decreased cTnI
or CK-MB readings.
i-STAT CHEM8+ cartridges require the use of:
1. whole blood collected in non-heparinized capillary tubes, evacuated
tubes, or syringes, as long as sample is tested immediately upon
draw,
2. heparinized whole blood collected in balanced heparin syringes or
capillary tubes, or
3. heparinized whole blood collected in evacuated tubes containing
lithium or sodium heparin, as long as the tubes are filled to capacity.
Fill Requirements
Fill blood collection tubes with
and without
anticoagulant and syringes with
anticoagulant to capacity. Incomplete filling of anticoagulated tubes and
syringes will cause higher heparin-to-blood ratios, which will decrease ionized
calcium results and may affect other results. Under filling blood collection
tubes with
and without
anticoagulant may also cause decreased
P
CO
2
, HCO
3
and TCO
2
results.
Partial-draw blood collection tubes (evacuated tubes that are adjusted to draw
less than the tube volume, e.g. a 5 mL tube with enough vacuum to draw only
3 mL), with
or without
anticoagulant, are not recommended for blood gas or
CHEM8+ cartridge analysis because of the potential for decreased
P
CO
2
,
HCO
3
and TCO
2
results. Care must also be taken to eliminate “bubbling” of
the sample with a pipette when filling a cartridge to avoid the loss of CO
2
in
the blood.
Mixing
Gently mix blood (whether anticoagulated or not) immediately to avoid
clotting. Invert a blood collection tube at least 10 times. Roll a syringe
vigorously between the palms for at least 5 seconds each in two different
directions, then invert the syringe repeatedly for at least 5 seconds, then
discard the first two drops of blood. Note that it may be difficult to properly
mix a sample in a 1.0 cc syringe.
Exposure to Air
Avoid exposing the sample to air when testing venous samples for ionized
calcium, pH,
P
CO
2
and TCO
2
. Test immediately if the sample is drawn into
a blood collection tube. Expel any air bubbles immediately if the sample is
drawn into a syringe or leave an air bubble next to the plunger and do not
allow it to move through the sample.
Summary of Contents for i-STAT 1
Page 1: ...i STAT 1 System Manual Rev Date 20 Jan 12 Art 714336 00L ...
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Page 627: ...PROCEDURE MANUAL FOR THE i STAT SYSTEM 6 REV DATE 16 Oct 12 ART 714446 00L ...
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