Abbott i-STAT 1, System Manual

Page: 170 / 658

10-4 Art: 714372-00L Rev. Date: 30-Jul-12
Time to Test
For the most accurate results, test samples immediately after drawing.
Samples for lactate must be tested immediately. Samples for pH,
P
CO
2
,
P
O
2
,
TCO
2
, and ionized calcium should be tested within 10 minutes. Other analytes
should be tested within 30 minutes.
If testing is not immediate, remix blood collection tubes by gentle inversion at
least 10 times. Roll syringes between the palms for at least 5 seconds each
in two different directions, then invert the syringe repeatedly for at least 5
seconds, and then discard the first two drops of blood. Blood in the tip of the
syringe may have been exposed to air and may not be homogenous with the
sample in the barrel of the syringe. Note It may be difficult to properly remix a
sample in a 1.0 cc syringe
ARTERIAL PUNCTURE - GENERAL
VENIPUNCTURE - COAGULATION TESTS
Blood Flow
Collection technique resulting in good blood flow must be used. Inadequate
blood flow may produce erroneous results.
Plastic The sample for testing should be drawn into a plastic collection device
(syringe or blood collection tube) containing no anticoagulant, clot
activators, or serum/plasma separators. Any transfer device (dispenser,
capillary tube, pipette or syringe)
must be plastic and must not contain
anticoagulant.
Samples collected into glass tubes or syringes, or in tubes containing
anticoagulants, activators, or separators cannot be used with the i-STAT
coagulation cartridges.
Note: CLSI guidelines recommend that the sample for coagulation testing be
the second or third tube drawn when using a blood collection system
(use a discard tube if this is the only sample being drawn) or be taken
from the second syringe if a double syringe technique is used for
drawing blood.
Time to Test
The sample must be immediately dispensed into the sample well of the
cartridge and the cartridge must be inserted immediately into an analyzer.
Repeat Test If a repeat measurement is needed, a fresh sample must be obtained.
Overview Arterial punctures are performed to access gas exchange status.
P
CO
2
,
P
O
2
, and pH values change with changes in ventilatory support at a
rate dependent on underlying conditions. Sample should be drawn after these
changes have stabilized.